Journal
BIOCHEMICAL JOURNAL
Volume 411, Issue -, Pages 97-105Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20070824
Keywords
erythropoietin; hypoxia-inducible factor 1 alpha (HIF-1 alpha) hydroxylase; intestinal trefoil factor; vascular endothelial growth factor (VEGF)
Categories
Ask authors/readers for more resources
HIF-1 (hypoxia-inducible factor 1) is a master regulator of cellular adaptive responses to hypoxia. The expression and transcriptional activity of the HIF-1 alpha subunit is stringently controlled by intracellular oxygen tension through the action of prolyl and asparaginyl hydroxylases. In the present study we demonstrate that PG (n-propyl gallate) activates HIF-1 and expression of its downstream target genes under normoxic conditions in cultured cells and in mice. The stability and transcriptional activity of HIF-1 alpha are increased by PG. PG treatment inhibits the interaction between HIF-1 alpha and VHL (von Hippel-Lindau protein) and promotes the interaction between HIF-1 alpha and p300, indicating that PG inhibits the activity of both prolyl and asparaginyl HIF-1 alpha hydroxylases. We conclude that PG activates HIF-1 and enhances the resultant gene expression by directly affecting the intracellular oxygen sensing system in vitro and in vivo and that PG represents a lead compound for the development of a non-toxic activator of HIF-1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available