Journal
SCIENCE
Volume 292, Issue 5521, Pages 1540-1543Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.292.5521.1540
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- NIGMS NIH HHS [GM-50526] Funding Source: Medline
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The Ran guanosine triphosphatase (GTPase) controls nucleocytoplasmic transport, mitotic spindle formation, and nuclear envelope assembly. These functions rely on the association of the Ran-specific exchange factor, RCC1 (regulator of chromosome condensation 1), with chromatin. We find that RCC1 binds directly to mononucleosomes and to histones H2A and H2B. RCC1 utilizes these histones to bind Xenopus sperm chromatin, and the binding of RCC1 to nucleosomes or histones stimulates the catalytic activity-of RCC1, We propose that the docking of RCC1 to H2A/H2B establishes the polarity of the Ran-CTP gradient that drives nuclear envelope assembly, nuclear transport, and other nuclear events.
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