Journal
FEBS LETTERS
Volume 497, Issue 2-3, Pages 141-147Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)02464-4
Keywords
Epstein-Barr virus; human monoclonal antibody; cold agglutinin; cord red cell; glycolipid
Ask authors/readers for more resources
Cord red cell membranes express many differentiation-related molecules. To study such molecules, we have established human cell lines, termed CL-1 and GL-2, by the Epstein-Barr virus transformation method, both of which produce monoclonal anti-i cold agglutinin [Y. Nagatsuka et al,, Immunol. Lett, 46 (1995) 93-100], Thin layer chromatography immunoblotting analysis revealed that these antibodies had broad specificities reacting with a variety of glycolipid antigens. Of the immunoreactive lipid antigens, a new phosphoglycerolipid containing glucose from human cord red cells was found. The isolated lipid was unstable to alkaline hydrolysis and contained glucose as a sole sugar, Secondary ion mass spectrum-collision-induced dissociation mass spectrometric analysis of this lipid gave the main molecular ion peak at m/z 885 corresponding to phosphatidylhexose. This antigen was susceptible to phospholipases A2, C and D but resistant to phosphatidylinositol-specific phospholipase C. Two-dimensional nuclear magnetic resonance spectroscopy confirmed that glucose is linked to the sn-glycerol 3-phosphate residue with a p-anomeric configuration, Based upon these combined results, we identified this lipid as phosphatidyl-beta -D-glucose. This is the first report showing the presence of the glucosylated glycerophospholipid in mammalian sources. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available