Journal
BIOCHEMICAL JOURNAL
Volume 411, Issue -, Pages 233-239Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20071296
Keywords
abscission; charged multivesicular body protein-3 (CHMP3); cytokinesis; endosome; endosomal sorting complexes required for transport (ESCRT); midbody
Categories
Ask authors/readers for more resources
In eukaryotic cells, the completion of cytokinesis is dependent on membrane trafficking events to deliver membrane to the site of abscission. Golgi and recycling endosomal-derived proteins are required for the terminal stages of cytokinesis. Recently, protein subunits of the ESCRT (endosomal sorting complexes required for transport) that are normally involved in late endosome to lysosome trafficking have also been implicated in abscission. Here, we report that a subunit, CHMP3 (charged multivesicular body protein-3), of ESCRT-III localizes at the midbody. Deletion of the C-terminal autoinhibitory domain of CHMP3 inhibits cytokinesis. At the midbody, CHMP3 does not co-localize with Rab11, suggesting that it is not present on recycling endosomes. These results combined provide compelling evidence that proteins involved in late endosomal function are necessary for the end stages of cytokinesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available