Journal
GENE
Volume 270, Issue 1-2, Pages 201-209Publisher
ELSEVIER
DOI: 10.1016/S0378-1119(01)00475-9
Keywords
alternating purine pyrimidine repeat; CRE/ATF; e-box; embryonal carcinoma cells
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Funding
- NCI NIH HHS [CA74771, CA79491, CA36727] Funding Source: Medline
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This report describes the isolation and characterization of the 5' flanking region of the murine transforming growth factor beta-2 (TGF-beta2) gene. A genomic clone containing the promoter region of the gene was isolated after screening a bacteriophage Pi genomic library. The resulting clone was sequenced and compared to promoters for the human and chicken TGF-beta2 genes. The sequence located near the transcription start site is highly conserved. It includes a TATA box, an E-box, and a largely conserved CRE/ATF site. A series of murine TGF-beta2 promoter/reporter constructs was generated to identify regulatory regions of the gene. As in the case of the human TGF-beta2 gene, sequences just upstream of the TATA box, including the CRE/ATF site, actively stimulate the murine TGF-beta2 promoter. However, unlike the human TGF-beta2 gene. the 5' flanking region of the murine TGF-beta2 gene contains a long alternating purine/pyrimidine repeat that unexpectedly exerts a strong positive effect on its promoter. This is of particular interest since alternating purine/pyrimidine repeats in other promoters have been observed to be inhibitory. (C) 2001 Elsevier Science B.V. All rights reserved.
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