Journal
BIOCHEMICAL GENETICS
Volume 49, Issue 9-10, Pages 656-664Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10528-011-9440-x
Keywords
APX; MnSOD; Synergistic expression
Funding
- Chinese Ministry of Education [307018]
- National Science and Technology Pillar Program [2007BAD37B06]
- Natural Science Foundation of Hubei Province [2007ABD003]
- National Natural Science Foundation of China [30771375]
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A novel ascorbate peroxidase (APX) cDNA was obtained from Nelumbo nucifera (Elian). The phylogenetic analysis indicated that N. nucifera APX grouped together with chloroplastic APX of high plants. The recombinant protein expressed by PET-30a vector showed APX activity (0.04 mM ascorbate min(-1) mg(-1) protein). The APX mRNA was expressed in young leaves, roots, terminal buds, and leaf-stalks. Synergistic expression of N. nucifera APX and MnSOD mRNA was indicated in the short-term response to mechanical wounding.
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