Journal
BIOCHEMICAL GENETICS
Volume 48, Issue 11-12, Pages 954-961Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10528-010-9377-5
Keywords
Chukar partridge; Genetic diversity; RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction); Khorasan-e-Razavi province; Gene flow
Funding
- agricultural faculty of Ferdowsi University of Mashhad, Iran
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RAPD markers were used to investigate population genetic parameters of an endangered partridge, Alectoris chukar, in four areas of Iran, as a part of a genetic conservation program. The aim of this study was to analyze the genetic similarity among these populations. Blood samples from 75 birds were used for DNA extraction and RAPD-PCR analysis of 67 loci, with 28 polymorphic bands (41.79%). The populations of Kalat-e-Nader and Mashhad were found to be closely related, as were the Torbat-e-Jaam and the Quchan populations. Mean heterozygosity for all populations was 0.4405 +/- A 0.0755. The results indicate that chukar partridge genetic diversity in Khorasan-e-Razavi province is sufficient and the amount of gene flow among populations is acceptable.
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