Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 90, Issue -, Pages 316-323Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2014.06.026
Keywords
Metabolic profiling analysis; Biodegradation; Chromatography; Microbial growth; Waste treatment; Pseudomonas sp.
Funding
- National Key Basic Research Program of China [2014CB745100]
- National Natural Science Foundation of China [20906070]
- Tianjin Research Program of Application Foundation and Advanced Technology [14JCQNJC06700]
- Seed Foundation of Tianjin University
- Program of Introducing Talents of Discipline to Universities [B06006]
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To investigate the enhancement of phenol on the biodegradation of 4-chlorophenol (4-cp), metabolic profiling approach was performed for the first time to analyze metabolite changes of Pseudomonas sp. cbp1-3 using single substrate (succinate, phenol, and 4-cp) and dual substrate (mixtures of phenol and 4-cp). Phosphoric acid, gamma-aminobutyric acid, 4-cp, 4-chlorocatechol, and catechol were shown to change significantly. Results indicated that phenols, especially 4-cp, depressed cell growth by inhibiting its primary metabolic pathway. In addition, the addition of phenol into the 4-cp-containing medium had a global influence on cells including the accumulation of amino acids, amines, saturated fatty acids, and monoacyl-glycerols as well as the concentration changes of-metabolite participating in phenols biodegradation, thus enhancing the degradation of 4-cp. This study provided novel insights into the biodegradation of mixed phenolic compounds and the method could be used to investigate the biodegradation of complicated multi-pollutants. (C) 2014 Elsevier B.V. All rights reserved.
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