Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 91, Issue -, Pages 92-98Publisher
ELSEVIER
DOI: 10.1016/j.bej.2014.08.002
Keywords
Actinobacillus succinogenes; Succinic acid; Bioprocess design; Bioreactors; Fermentation; Immobilized cells
Funding
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- 111 Project [111-2-06]
- Jiangsu province Collaborative Innovation Center for Advanced Industrial Fermentation industry development program
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Succinic acid (SA) was produced from Actinobacillus succinogenes with high cell density by continuous fermentation using fibrous bed bioreactor (FBB). The effects of feeding glucose concentration, dilution rate, and pH on continuous production of SA were examined to achieve an efficient and economical bioprocess. The optimum feeding glucose concentration, dilution rate, and pH were 80 g/L, 0.051/h, and 6.0-6.5, respectively. A SA concentration of 55.3 +/- 0.8 g/L, productivity of 2.77 +/- 0.04 g/L/h, and yield of 0.8 +/- 0.02 g/g were obtained, and the continuous fermentation exhibited long-term stability for as long as 18 days (440 h) with no obvious fluctuations in both SA and biomass levels. The Jerusalimsky equation for the specific rate of SA production presented the inhibition phenomenon of the product, demonstrating that 60 g/L SA might be a critical concentration in this continuous FBB system. The results obtained could be beneficial for future fermentor designs and improvements in SA production. (C) 2014 Elsevier B.V. All rights reserved.
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