The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4

There is a growing body of evidence that the ambr (TM) workstation from TAP Biosystems performs well in terms of helping to select appropriate clones for scale-up studies. Here we have investigated the physical characteristics of this microscale bioreactor system and found that these are quite different from those that exist in larger scale stirred bioreactors. For example, the flow regime in the ambr (TM) vessel is transitional rather than turbulent and the sparged air/oxygen superficial gas velocity is relatively very low whilst the specific power input is much higher (similar to 400 W/m(3)) when compared to that used at larger scales (typically similar to 20 W/m(3)). This specific power input is necessary in order to achieve k(L)a values sufficiently high to satisfy the oxygen demand of the cells and control of dO(2). In line with other studies, we find that the culture of CHO cells in a 15 mL ambr (TM) bioreactor gave similar cell growth and productivity to that achieved in a 5 L stirred bioreactor whilst the results from shake flasks were significantly different. Given the differences in physical characteristics between the ambr (TM) and larger stirred bioreactors, we suggest that this similarity in biological performance is due to their similar control capabilities and the 'equivalence of the stress parameters' across the scales when compared with shake flasks. (C) 2013 Elsevier B.V. All rights reserved.