Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 72, Issue -, Pages 96-101Publisher
ELSEVIER
DOI: 10.1016/j.bej.2013.01.006
Keywords
Oxalate oxidase; Active packaging; Oxygen scavenger; Oxalic acid; Latex; Entrapment
Funding
- Knowledge Foundation (KK-stiftelsen)
- Knut and Alice Wallenberg Foundation
- Bio4Energy program
Ask authors/readers for more resources
Oxalate oxidase has potential to act as an oxygen scavenger in active packaging to increase the shelf-life of food and beverages, while simultaneously producing the protective packaging gas carbon dioxide. This study shows that oxalate oxidase from barley can be immobilized with retained catalytic activity through entrapment in a latex polymer matrix. Conditions for formation of film containing oxalate oxidase have been evaluated as well as effects of storage and latex on enzyme activity, migration of enzyme in films, and the ability of the latex films to resist higher temperatures. Drying of enzyme-containing latex films at 75 degrees C prior to conditioning at 30 degrees C resulted in higher activity than drying solely at 30 degrees C, or drying at 95 degrees C or 105 degrees C followed by conditioning at 30 degrees C. Storage of films in air at 4 degrees C for 14 days did not negatively affect the enzymatic activity. Inclusion of catalase in films with oxalate oxidase effectively prevented release of hydrogen peroxide. The results suggest that the immobilized enzyme can successfully be used both as an oxygen scavenger and as an oxalic-acid scavenger. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available