Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 72, Issue -, Pages 18-23Publisher
ELSEVIER
DOI: 10.1016/j.bej.2012.12.010
Keywords
CLEA; Lipase; Layered CLEAs; Recovered activity
Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas [CONICET-PIP 112-200801-01856]
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT Redes2006 729/06]
- Universidad Nacional del Sur (Argentina) [PGI 24/M108-UNS]
Ask authors/readers for more resources
Cross-linked enzyme aggregates (CLEAs) of lipase from Thermomyces lanuginosa (TLL) were synthesized using (NH4)(2)SO4 as precipitant and glutaraldehyde as cross-linking agent. CLEAs were assayed for their hydrolytic activity in a reaction performed in an emulsioned medium. The effects of the amount of precipitant, cross-linker, and different additives such as protein cofeeder, oleic acid, n-heptane, sodium dodecyl sulfate (SDS), polyethylenglicol (PEG) and ethylendiamine were studied at selected ratios with respect to ILL mass. Traditional non-layered CLEAs of TLL showed recovered activities between 3 and 31% when compared with native lipase. Novel TLL layered CLEAs consisting of a protein cofeeder core and successive layers of target lipase showed an important increase in their retained activity. The highest recovered activity was found for the one-layered non-additivated CLEAs of TLL which showed a recovered activity of 75%. (C) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available