Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 78, Issue -, Pages 24-31Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bej.2012.11.017
Keywords
Microalgae; Scenedesmus obliquus; Lutein; Extraction kinetics; Bioseparations; Purification
Funding
- Taiwan's Stone & Resource Industry RD Center [SRDC-601-A1-180]
- Taiwan's National Science Council [101-3113-E-006-015, 101-3113-P-110-002]
- ATU plan for National Chung Hsing University
- National Cheng Kung University
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This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24 h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or -20 degrees C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value. (c) 2012 Elsevier B.V. All rights reserved.
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