Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 65, Issue -, Pages 1-9Publisher
ELSEVIER
DOI: 10.1016/j.bej.2012.03.009
Keywords
Pichia pastoris; Rhizopus oryzae; Recombinant lipase; Flavor synthesis; Ethyl butyrate; Immobilized lipase
Funding
- Spanish Ministry of Science and Innovation [CTQ2010-15131]
- Reference Network in Biotechnology (XRB) (Generalitat de Catalunya) [2009-SGR-281]
- FPU of the Spanish Ministry of Education and Science
Ask authors/readers for more resources
A recombinant Rhizopus oryzae lipase expressed in Pichia pastoris has been immobilized in three different kinds of supports: EP100, Eupergite (R) CM and Octadecyl-Sepabeads. These immobilized derivatives have been used to catalyze the synthesis of ethyl butyrate. The effect of different parameters on initial reaction rate and yield has been studied for each biocatalyst. The variables selected for the study were: temperature, water content, shaking speed, enzyme loading, type of solvent and substrates molar ratio. The results showed the EP100 derivative as the best choice in terms of initial rate and yield but, when the residual activity as well as the reutilization capacity was studied. Octadecyl-Sepabeads exhibits the highest stability, so it was finally chosen as the best biocatalyst. (c) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
