Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 50, Issue 1-2, Pages 29-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2010.03.001
Keywords
Nanofiltration; Enzyme immobilization; Oligosaccharides synthesis; Saccharides fractionation
Funding
- ICEMS
- Institute of Materials and Surfaces Science and Engineering, Portugal
- European Union [E07D402537BR]
Ask authors/readers for more resources
Continuous galacto-oligosaccharides (GOS) synthesis by bead-immobilized beta-galactosidase and nanofiltration fractionation of derived sugar mixtures by cellulose acetate membranes are investigated. Upon enzyme adsorption assays (8 mL/g of solution/resin ratio, 150 mg/mL of initial enzyme concentration and 14 +/- 1.4 mg protein/g resin), a Langmuir-type isotherm is observed. Enzyme concentration of 10 U/mL, selected in batch reactor experiments, is used for continuous GOS production carried out at lactose concentrations of 150 g/L and 300 g/L, and at 40 degrees C. Total dissolved solids concentration influences both reaction and saccharide mixtures fractionation. All observed sugar retention coefficients, mainly for monosaccharides, increase with transmembrane pressure and a gap between the retention values of synthesized disaccharides and lactose (87% versus 77%, respectively, for 2 bar/40 degrees C) is displayed as a function of the effective transmembrane pressure and total sugars concentration. At 150 g/L total sugars in feed, trisaccharides are totally retained. Doubling sugars concentration, trisaccharides retentions decreased for all applied pressures, reaching 90%. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available