Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 46, Issue 1, Pages 61-64Publisher
ELSEVIER
DOI: 10.1016/j.bej.2009.03.010
Keywords
Solid state rotary drum bioreactor; Erlenmeyer flask; Oil palm biomass; Cellulase enzyme; Trichoderma harzianum; Solid waste treatment
Funding
- Research Management Center (RMC)
- International Islamic University Malaysia (IIUM) [LT 33]
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Bioconversion of oil palm lignocellulosic biomass in the form of empty fruit bunches (EFB) as the major substrate into cellulase enzyme was studied in the laboratory. A fungal strain, Trichoderma harzianum T2008 was used to evaluate the solid state bioconversion of EFB for cellulase production. The study was conducted in two systems: an Erlenmeyer flask (EF, 500 mL) and a horizontal rotary drum bioreactor (RDB, 50 L), designed and fabricated locally. The highest cellulase activity on the fourth day of fermentation in the EF was 8.2 filter paper activity (FPA)/gram dry solids (gds) of EFB, while its activity from the RDB was 10.1 FPA/gds on the second day of fermentation. Glucosamine, reducing sugar and pH were also determined to evaluate the substrate uptake and growth conditions during the entire fermentation period. (C) 2009 Elsevier B. V. All rights reserved.
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