Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 46, Issue 2, Pages 199-204Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2009.05.012
Keywords
Hemicellulose; Biomass; Lignocellulose; Sugarcane bagasse; Extraction; Biopolymers
Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP [06/03564-9, 08/03204-8]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [08/03204-8, 06/03564-9] Funding Source: FAPESP
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A process has been elaborated for one-step low lignin content sugarcane bagasse hemicellulose extraction using alkaline solution of hydrogen peroxide. To maximize the hemicellulose yields several extraction conditions were examined applying the 2(4) factorial design: H2O2 concentration from 2 to 6% (w/v), reaction time from 4 to 16 h, temperature from 20 to 60 degrees C, and magnesium sulfate absence or presence (0.5%, w/v). This approach allowed selection of conditions for the extraction of low and high lignin content hemicellulose. At midpoint the yield of hemicellulose was 94.5% with more than 88% of lignin removed. Lignin removal is suppressed at low extraction temperatures and in the absence of magnesium sulfate. Hemicellulose in 86% yield with low lignin content (5.9%) was obtained with 6% H2O2 treatment for 4 h and 20 degrees C. This hemicellulose is much lighter in color than samples obtained at the midpoint condition and was found suitable for subsequent enzymatic hydrolysis. (C) 2009 Elsevier B.V. All rights reserved.
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