Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 41, Issue 3, Pages 234-240Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2008.04.020
Keywords
amino acids; glutathione (GSH); Candida utilis; optimization; fermentation; glucose
Funding
- Chinese National 863 Project [2006AA10Z313]
- Innovation Fund of Medium or Small Science and Technology Enterprise [06026213201074]
- National Science Fund [20625619]
- 973 Project [2007CB714306]
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Strategy of precursor amino acids addition for glutathione (GSH) synthesis was optimized in this study. The addition of cysteine and glycine showed a significant effect on GSH accumulation. Cysteine increased intracellular GSH content greatly but inhibited cell growth at the same time. A two-step amino acids addition strategy was developed. First. 6 mmol/L glycine was added at 4 h, and a mixture of glutamic acid. glycine and cysteine was added at 12 h at stationary phase of cell growth. The optimum concentrations of three amino acids (glutamic acid. 6 mmol/L; glycine. 5.5 mmol/L; cysteine. 6.5 mmol/L) were obtained by response surface methodology. With the two-step addition strategy. biomass and GSH yield reached 11.65 g/L and 241.4 mg/L in flask experiments, increased by 10.5% and 69.5%, respectively. as compared with the control without amino acids addition. By applying this amino acid addition strategy further to batch fermentation in 7 L fermentor, GSH yield reaches 383.3 mg/L, which is 78.1% higher than the control. (C) 2008 Elsevier B.V. All rights reserved.
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