Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 445, Issue 4, Pages 791-794Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.01.141
Keywords
CRISPR/Cas9; Genome-modified animals; Nickase; Off-target
Categories
Funding
- Japan Society for the Promotion of Science [25892010, 20188858]
- Grants-in-Aid for Scientific Research [25892010, 25252056] Funding Source: KAKEN
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The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted genome-modified animals. However, this system is limited by the risk of off-target mutations. Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate region deleted mice and targeted knock-in mice without off-target mutations. (c) 2014 The Authors. Published by Elsevier Inc.
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