Journal
JOURNAL OF GENERAL VIROLOGY
Volume 82, Issue -, Pages 1457-1463Publisher
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/0022-1317-82-6-1457
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Translation elongation factor 1 delta (EF-1 delta) is hyperphosphorylated in various mammalian cells infected with alpha-, beta- and gammaherpesviruses and EF-1 delta modification is mediated by viral protein kinases, including UL13 of herpes simplex virus type 1 and UL97 of human cytomegalovirus. In this study, the following is reported, (i) BGLF4 encoded by the prototype gammaherpesvirus Epstein-Barr virus was purified as a fusion protein that was labelled with [gamma-P-32]ATP and labelling was eliminated by phosphatase. (ii) The ratio of the hyperphosphorylated form of human EF-1 delta was increased both in Sf9 cells after infection with baculoviruses expressing GST-BGLF4 fusion proteins and in COS-7 cells after transfection with a BGLF4 expression plasmid, These results indicate that purified BGLF4 possesses protein kinase activity and mediates EF-1 delta hyperphosphorylation. These data also support the hypothesis that the protein kinases that are conserved by herpesviruses universally mediate EF-1 delta modification in mammalian cells.
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