Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 445, Issue 4, Pages 694-701Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2013.12.070
Keywords
Epithelial ovarian cancer; Exosomes Mass spectrometry; Proteomics; Trifluoroethanol
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Funding
- Canadian Institute of Health Research [MOP-93772]
- Ontario Ministry of Health and Long Term Care
- Canadian Research Chairs Program
- Medical Biophysics Excellence Award
- Kristi Piia Callum Memorial Fellowship
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Molecular communication between cancer cells and its stromal microenvironment is a key factor for cancer progression. Alongside classic secretory pathways, it has recently been proposed that small membranous vesicles are alternative mediators of intercellular communication. Exosomes carry an effector-rich proteome with the ability to modulate various functional properties of the recipient cell. In this study, exosomes isolated from four epithelial ovarian cancer cell lines (OVCAR3, OVCAR433, OVCAR5 and SKOV3) were characterized using mass spectrometry-based proteomics. Using an optimized workflow consisting of efficient exosome solubilization and the latest generation of proteomic instrumentation, we demonstrate improved detection depth. Systematic comparison of our cancer cell line exosome proteome against public data (Exocarta) and the recently published NCI 60 proteome revealed enrichment of functional categories related to signaling biology and biomarker discovery. (C) 2014 Elsevier Inc. All rights reserved.
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