Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 452, Issue 4, Pages 888-894Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.08.116
Keywords
Antioxidant activity; Chromatography; Duck egg white protein; Bioactive peptide; Isolation and purification
Categories
Funding
- National Natural Science Foundation of China [20906031, 31270636]
- Natural Science Foundation of Guangdong Province [S2013010012486]
- Self-determined research funds of SCUT from the college' basic research and operation of Ministry of Education of China [2013ZM0065, 2013ZM0044, 2013ZZ0068]
- Project of Department of Education of Guangdong Province [2012CXZD0009, 2012KJCX0006]
- Program for New Century Excellent Talents in University [NCET-12-0192]
Ask authors/readers for more resources
The hydrolysate from duck egg white protein (DEWP) prepared by SEEP-Alcalase at degree of hydrolysis (DH) value of 21% (namely HSA(21)) exhibited high antioxidant capacity in different oxidation systems. A consecutive chromatographic method was then developed for separation and purification of HSA(21), including ion-exchange chromatography, macroporous adsorption resin (MAR) and gel filter chromatography. The final peptides P21-3-75-B were obtained with significantly enhanced antioxidant activity (p < 0.05). It was further confirmed that the product mainly consisted of five oligopeptides (Mr: 202.1, 294.1, 382.1, 426.3, and 514.4 Da). Furthermore, the antioxidant activity of P21-3-75-B kept stable after in vitro digestive simulation. Antioxidant capacity of the purified peptides was closely related to the molecular mass, hydrophobic amino acid residues, acidic amino acid and some antioxidant amino acids. This research provided a valuable route for producing new natural-source peptides with strong antioxidant capacity and high nutritious value for our daily intake. (C) 2014 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available