Journal
MYCORRHIZA
Volume 11, Issue 2, Pages 89-94Publisher
SPRINGER-VERLAG
DOI: 10.1007/s005720100103
Keywords
Tuber indicum; Tuber melanosporum; internal transcript spacer sequence analysis polymerase chain reaction; molecular markers
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Although authorized for the French market, the Asiatic black truffle, Tuber indicum, may potentially be sold as I: melanosporum, the Perigord black truffle, due to difficulties in distinguishing between them by morphological criteria. A molecular method using a DNA extraction kit and specific primers based on the interspecific polymorphism of the internal transcript spacer DNA region has allowed us to amplify I: indicum samples (mycorrhizas, fresh, dried, and frozen ascocarps) and even to detect as little as 1% of Asiatic truffle in a mixture of ground ascocarps or mycorrhizas containing ir: melanosporum. Our process is specific, sensitive, convenient and quick, so it could be used for the rapid detection of I: indicum in non-cooked food products and commercialized mycorrhizal seedlings to avoid the ecological damage which would result from its accidental introduction.
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