Journal
BLOOD
Volume 97, Issue 11, Pages 3333-3341Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood.V97.11.3333
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Funding
- NCI NIH HHS [CA-42551] Funding Source: Medline
- NIAID NIH HHS [5T32-AI-07290] Funding Source: Medline
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It has been proposed that there are at least 2 classes of dendritic cells (DCs), CD8 alpha (+) DCs derived from the lymphoid lineage and CD8 alpha (-) DCs derived from the myeloid lineage. Here, the abilities of lymphoid- and myeloid-restricted progenitors to generate DCs are compared, and their overall contributions to the DC compartment are evaluated. It has previously been shown that primitive myeloid-committed progenitors (common myeloid progenitors [CMPs]) are efficient precursors of both CD8 alpha (+) and CD8 alpha (-) DCs in vivo. Here it is shown that the earliest lymphoid-committed progenitors (common lymphoid progenitors [CLPs]) and CMPs and their progeny granulocyte-macrophage progenitors (GMPs) can give rise to functional DCs in vitro and in vivo. CLPs are more efficient in generating DCs than their T-lineage descendants, the early thymocyte progenitors and pro-T cells, and CMPs are more efficient DC precursors than the descendant GMPs, whereas pro-B cells and megakaryocyte-erythrocyte progenitors are incapable of generating DCs, Thus, DC developmental potential is preserved during T- but not B-lymphoid differentiation from CLP and during granulocyte-macrophage but not megakaryocyte-erythrocyte development from CMP In vivo reconstitution experiments show that CLPs and CMPs can reconstitute CD8 alpha (+) and CD8 alpha (-) DCs with similar efficiency on a per cell basis, However, CMPs are 10-fold more numerous than CLPs, suggesting that at steady state, CLPs provide only a minority of splenic DCs and approximately half the DCs in thymus, whereas most DCs, including CD8 alpha (+) and CD8 alpha (-) subtypes, are of myeloid origin. (Blood, 2001;97:3333-3341) (C) 2001 by The American Society of Hematology.
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