Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 449, Issue 4, Pages 466-470Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.05.042
Keywords
Extracellular signal-regulated kinase-5; (ERK5) pathway; Tumor necrosis factor-alpha (TNF-alpha); Nucleus pulposus; Nucleus pulposus degeneration
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Funding
- Foundation Medical and Health Key Project of Guangzhou City [2009-zdi-04]
- Traditional Chinese Medicine Project of Guangzhou City [2012A011030040]
- Guangdong Provincial Natural Science Foundation of China [10151022001000005, S2011010000910]
- Natural Science Foundation of China (NSFC) [81228013]
- [2012A011030012]
- [2013A011030005]
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Extracellular-signal-regulated kinase 5 (ERK5) is a member of the mitogen-activated protein kinase (MAPK) family and regulates a wide variety of cellular processes such as proliferation, differentiation, necrosis, apoptosis and degeneration. However, the expression of ERK5 and its role in degenerated human nucleus pulposus (NP) is hitherto unknown. In this study, we observed the differential expression of ERK5 in normal and degenerated human nucleus pulposus tissues by using immunohistochemical staining and Western blot. Treatment of NP cells with Pro-inflammatory cytokine, TNT-alpha decreased ERK5 gene expression as well as NP marker gene expression; including the type II collagen and aggrecan. Suppression of ERK5 gene expression in NP cells by ERK5 siRNA resulted in decreased gene expression of type II collagen and aggrecan. Furthermore, inhibition of ERK5 activation by BIX02188 (5 mu M) decreased the gene expression of type II collagen and aggrecan in NP cells. Our results document the expression of ERK5 in degenerated nucleus pulposus tissues, and suggest a potential involvement of ERK5 in human degenerated nucleus pulposus. (C) 2014 Elsevier Inc. All rights reserved.
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