Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 449, Issue 2, Pages 248-255Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.05.018
Keywords
Carboxylation; E-Box motif; CG dinucleotide; Basic-helix-loop-helix; DNA binding; Tcf3 vertical bar Ascl1 heterodimer
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Funding
- National Cancer Institute, NIH, Bethesda, USA
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Three oxidative products of 5-methylcytosine (5mC) occur in mammalian genomes. We evaluated if these cytosine modifications in a CG dinucleotide altered DNA binding of four B-HLH homodimers and three heterodimers to the E-Box motif CGCAG vertical bar GTG. We examined 25 DNA probes containing all combinations of cytosine in a CG dinucleotide and none changed binding except for carboxylation of cytosine (5caC) in the strand CGCAG vertical bar GTG. 5caC enhanced binding of all examined B-HLH homodimers and heterodimers, particularly the Tcf3 vertical bar Ascl1 heterodimer which increased binding similar to 10-fold. These results highlight a potential function of the oxidative products of 5mC, changing the DNA binding of sequence-specific transcription factors.
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