4.6 Article

Tetraspanin CD9 modulates human lymphoma cellular proliferation via histone deacetylase activity

Journal

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.04.046

Keywords

Tetraspanin; CD9; Lymphoma; Histone deacetylase; Cell proliferation

Funding

  1. Vascular Biology Center of Excellence Support Fund
  2. University of Tennessee Health Science Center
  3. American Heart Association (AHA) Scientist Development Grant
  4. AHA Southeast Affiliate Grant-in-Aid
  5. AHA Predoctoral Fellowship
  6. Patricia Kouns Fellowship Fund

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Non-Hodgkin Lymphoma (NHL) is a type of hematological malignancy that affects two percent of the overall population in the United States. Tetraspanin CD9 is a cell surface protein that has been thoroughly demonstrated to be a molecular facilitator of cellular phenotype. CD9 expression varies in two human lymphoma cell lines, Raji and BJAB. In this report, we investigated the functional relationship between CD9 and cell proliferation regulated by histone deacetylase (HDAC) activity in these two cell lines. Introduction of CD9 expression in Rail cells resulted in significantly increased cell proliferation and HDAC activity compared to Mock transfected Rail cells. The increase in CD9-Raji cell proliferation was significantly inhibited by HDAC inhibitor (HDACi) treatment. Pretreatment of BJAB cells with HDAC inhibitors resulted in a significant decrease in endogenous CD9 mRNA and cell surface expression. BJAB cells also displayed decreased cell proliferation after HDACi treatment. These results suggest a significant relationship between CD9 expression and cell proliferation in human lymphoma cells that may be modulated by HDAC activity. (C) 2014 Elsevier Inc. All rights reserved.

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