Journal
JOURNAL OF FLUORESCENCE
Volume 11, Issue 2, Pages 101-107Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1023/A:1016673300913
Keywords
lanthanides; multiphoton excitation; ligands; europium terbium; sensitizers; photostability
Funding
- NCRR NIH HHS [P41 RR008119] Funding Source: Medline
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We describe multiphoton excitation of the lanthanides europium (Eu3+) and terbium (Tb3+) when these ions are complexed with nucleic acids, proteins, and fluorescent chelators. In all cases excitation occurs by multiphoton absorption of the sensitizers. For the nucleotide GDP and an oligonucleotide with several guanines, the sensitized emission of Tb3+ excited at 776 rim indicated a three-photon process. For Tb3+ bound to the wild-type troponin C and a single tryptophan mutant (26W), excitation at 794 nm was also close to a three-photon process. For lanthanide chelators containing various sensitizers, we observed three-photon excitation in the case of methyl anthranilate, a mixuture of two-and three-photon excitation for carbostyril 124, and a two-photon process with a coumarin derivative. In the case of coumarin-sensitized emission of Eu3+ varied from a two- to a three-photon process at wavelengths ranging from 780 to 880 unit. The sensitized luminescence also shows significantly higher photostability compared to the fluorescence from the organic fluorophores alone. These results suggest the use of multiphoton-induced sensitized lanthanide fluorescence in biochemistry and cellular imaging.
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