Journal
FEBS LETTERS
Volume 498, Issue 1, Pages 57-61Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)02495-4
Keywords
acylation; palmitoylation; virus fusion; membrane fusion liposome
Funding
- NHLBI NIH HHS [HL 16660] Funding Source: Medline
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The envelope glycoproteins E1 and E2 of Sindbis virus are palmitoylated at cysteine residues within their transmembrane domains (E1 at position 430, and E2 at positions 388 and 390), Here, we investigated the in vitro membrane fusion activity of Sindbis virus variants (derived from the Tote 1101 infectious clone), in which the E1 C430 and/or E2 C388/390 residues had been substituted for alanines, Both the E1 and E2 mutant viruses, as well as a triple mutant virus, fused with liposomes in a strictly low-pH-dependent manner, the fusion characteristics being indistinguishable from those of the parent Tote 1101 virus. These results demonstrate that acylation of the transmembrane domain of Sindbis virus E1 and E2 is not required for expression of viral membrane fusion activity. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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