Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 19, Issue 2, Pages 129-135Publisher
INT SOC PLANT MOLECULAR BIOLOGY
DOI: 10.1007/BF02772155
Keywords
aquaporin; epidermis; guard cell; in situ hybridization; Vicia faba
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Because the epidermis of V. faba L. leaves easily can be peeled into strips of one cell layer, we developed a simple method of in situ hybridization using epidermal peels as a substitute for paraffin, resin and cryosections. Our method sufficiently detected the expression of broad bean aquaporin 1 in guard cells. RT-PCR revealed higher expression of aquaporins (AQPs) in guard cells compared to other leaf cell types; this indicates the importance of AQP for bulk water flow across guard cell membranes and, therefore, for stomatal movements.
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