Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 421, Issue 2, Pages 296-300Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2012.04.003
Keywords
Phospholipid; PgsA; pssA; Rcs; Cpx; Outer membrane lipoprotein
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Funding
- Grants-in-Aid for Scientific Research [22570001] Funding Source: KAKEN
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The pgsA gene encodes the enzyme for the committed step in the synthesis of acidic phospholipids in Escherichia coli, and the pssA gene does the same for zwitterionic phospholipid. It has been reported that the Rcs and Cpx phosphorelay signal transduction systems are activated in pgsA- and pssA-defective mutants, respectively. In this study, we show that the Cpx system is activated also in a pgsA mutant, whereas the Rcs system was not activated in a pssA mutant. Lack of phosphatidylglycerol in pgsA mutants causes inadequate modification of lipoproteins, resulting in poor localization to the outer membrane. The outer membrane lipoprotein RcsF is necessary for the response of the Rcs system to various stimuli, and Rcs activation in pgsA mutants involves inner membrane mislocalization of this lipoprotein. The outer membrane lipoprotein NlpE, however, while necessary for the surface adhesion-induced Cpx response, was not involved in Cpx activation in the pgsA mutant. (C) 2012 Elsevier Inc. All rights reserved.
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