Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 21, Issue 12, Pages 3926-3934Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.21.12.3926-3934.2001
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Funding
- NCI NIH HHS [CA09302, T32 CA009302] Funding Source: Medline
- NIDDK NIH HHS [R01 DK058187, DK58187, DK55569] Funding Source: Medline
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We previously established that the phage phi C31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. We show here that phage attP sites inserted at various locations in human and mouse chromosomes serve as efficient targets for precise site-specific integration. Moreover, we characterize native pseudo attP sites in the human and mouse genomes that also mediate efficient integrase-mediated integration. These sites have partial sequence identity to attP. Such sites form naturally occurring targets for integration. This phage integrase-mediated reaction represents an effective site specific integration system for higher cells and may be of value in gene therapy and other chromosome engineering strategies.
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