4.6 Article

Protective effect of L-theanine on carbon tetrachloride-induced acute liver injury in mice

Journal

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2012.05.022

Keywords

L-theanine; Carbon tetrachloride; Acute liver injury; Oxidative stress; Inflammatory response; Apoptosis

Funding

  1. National Nature Science Foundation of China [81072433, 81172798, 31071000, J1103512]
  2. Priority Academic Program Development of Jiangsu Higher Education Institutions [164320H106]

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We studied effects of L-theanine, a unique amino acid in tea, on carbon tetrachloride (CCl4)-induced liver injury in mice. The mice were pre-treated orally with L-theanine (50, 100 or 200 mg/kg) once daily for seven days before CCl4 (10 ml/kg of 0.2% CCl4 solution in olive oil) injection. L-theanine dose-dependently suppressed the increase of serum activity of ALT and AST and bilirubin level as well as liver histopathological changes induced by CCl4 in mice. L-theanine significantly prevented CCl4-induced production of lipid peroxidation and decrease of hepatic GSH content and antioxidant enzymes activities. Our further studies demonstrated that L-theanine inhibited metabolic activation of CCl4 through down-regulating cytochrome P450 2E1 (CYP2E1). As a consequence, L-theanine inhibited oxidative stress-mediated inflammatory response which included the increase of TNF-alpha and IL-1 beta in sera, and expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in livers. CCl4-induced activation of apoptotic related proteins including caspase-3 and PARP in mouse livers was also prevented by L-theanine treatment. In summary, L-theanine protects mice against CCl4-induced acute liver injury through inhibiting metabolic activation of CCl4 and preventing CCl4-induced reduction of anti-oxidant capacity in mouse livers to relieve inflammatory response and hepatocyte apoptosis. (C) 2012 Elsevier Inc. All rights reserved.

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