Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 426, Issue 1, Pages 7-11Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2012.07.116
Keywords
Magnetotactic bacteria; Bacterial magnetic particles; Protein display technology; Mms13; Human protein expression
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Funding
- Scientific Research for the Ministry of Education, Culture, Sports, Science and Technology of Japan [23226016]
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Grants-in-Aid for Scientific Research [23226016] Funding Source: KAKEN
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Biologically synthesized magnetic particles by magnetotactic bacteria (BacMPs) have promising potential in the area of functional protein display technology for various biotechnological and biomedical applications. Functional proteins fused with an anchor protein, Mms13, have been demonstrated to be an effective and stable method for the display of functional proteins on BacMPs. However, the expression of some human proteins is relatively low. Useful host strains of Escherichia coli have been developed for the enhanced expression of recombinant proteins using a genetic engineering approach. To improve human protein expression level on BacMPs in Magnetospirillum magneticum AMB-1, a mutant strain with a deleted native mms13 gene (Delta mms13 strain) was established and evaluated for effective functional protein display technology. As a result, the Delta mms13 strain synthesized BacMPs with significantly improved expression of two human proteins, thyroid-stimulating hormone receptor (TSHR) and the class II major histocompatibility complex (MHC II) molecules. The Delta mms13 strain could therefore be an effective strain for the display of other important human proteins on BacMPs and may be useful for further applications. (C) 2012 Elsevier Inc. All rights reserved.
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