Journal
JOURNAL OF APPLIED PHYSIOLOGY
Volume 90, Issue 6, Pages 2386-2402Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jappl.2001.90.6.2386
Keywords
formalin test; spinal sensitization; pain models; flinching behavior
Categories
Ask authors/readers for more resources
The biphasic display of paw-flinch behavior in the rat after injection of formalin into the dorsum of the hind paw is used for the screening of anti-hyperalgesic agents. Described and characterized here is a less labor-intensive system for counting flinch activity by detecting movement of a small metal band placed on the formalin-injected paw. A signal is generated as the band breaks the electromagnetic field of a loop antenna located under the rat and processed through an algorithm that determines flinch activity using 1) amplitude, 2) zero-voltage crossing, and 3) signal duration. Flinches are summed and stored over a selected collection interval throughout the assay for later analysis. Studies have validated the measures with respect to 1) system stability over time; 2) system-to-practiced observer correlation on flinch detection, r(2) = 0.94; 3) system variables including time of day, sex, age, and body weight; and 4) 50% effective dose values similar to those previously reported for intrathecal morphine and the NMDA antagonist MK-801.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available