Journal
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Volume 280, Issue 6, Pages H2489-H2495Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.2001.280.6.H2489
Keywords
contractile dysfunction; polymorphonuclear leukocytes; polymorphonuclear neutrophil adherence
Funding
- NHLBI NIH HHS [HL-07599] Funding Source: Medline
- NIGMS NIH HHS [GM-45434] Funding Source: Medline
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Caveolin-1 is a protein constituent of cell membranes. The caveolin-1 scaffolding region (residues 82-101) is a known inhibitor of protein kinase C. Inhibition of protein kinase C results in maintained nitric oxide (NO) release from the endothelium, which attenuates cardiac dysfunction after ischemia-reperfusion (I/R). Therefore, we hypothesized that the caveolin-1 scaffolding region of the molecule, termed caveolin-1 peptide, might attenuate postischemia polymorphonuclear neutrophil (PMN)-induced cardiac dysfunction. We examined the effects of caveolin-1 peptide in isolated ischemic (20 min) and reperfused (45 min) rat hearts reperfused with PMNs. Caveolin-1 peptide (165 or 330 mug) given intravenously 1 h before I/R significantly attenuated postischemic PMN-induced cardiac dysfunction, as exemplified by left ventricular developed pressure (LVDP) (P< 0.01) and the maximal rate of develped pressure (+dP/dt(max))( P< 0.01), compared with I/R hearts obtained from rats given 0.9% NaCl. In addition, caveolin-1 peptide significantly reduced cardiac PMN infiltration from 195 +/- 5 PMNs/mm(2) in untreated hearts to 103 +/- 5 and 60 +/- 5 PMNs/mm(2) in hearts from 165 and 330 mg caveolin-1 peptide-treated rats, respectively (P< 0.01). PMN adherence to the rat coronary vasculature was also significantly reduced in rats given either 165 or 330 mg caveolin-1 peptide compared with rats given 0.9% NaCl (P< 0.01). Moreover, caveolin-1 peptide-treated rat aortas exhibited a 2.2-fold greater basal release of NO than vehicle-treated aortas (P< 0.01), and this was inhibited by N-G-nitro-L-arginine methyl ester. These results provide evidence that caveolin-1 peptide significantly attenuated PMN-induced post-I/R cardiac contractile dysfunction in the isolated perfused rat heart, probably via enhanced release of endothelium-derived NO.
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