Journal
JOURNAL OF VIROLOGY
Volume 75, Issue 11, Pages 5421-5424Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.75.11.5421-5424.2001
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The factors present in serum and plasma samples of human immunodeficiency virus (HIV)-infected patients that are responsible for the neutralization of four HIV type 1 (HIV-1) primary isolates in vitro have been analyzed. Purification of immunoglobulins (Ig) by affinity chromatography showed that the activities were mostly attributable to Ige and less frequently to IgA, For two samples, we have shown that the high-level and broad-spectrum inhibitory activity was essentially caused by non-Ig factors interfering with the measurement of antibody-specific neutralizing activity.
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