Journal
ANALYTICA CHIMICA ACTA
Volume 436, Issue 1, Pages 87-101Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(01)00887-X
Keywords
antibody; triazine; pesticide; immunoassay; atrazine; simazine; terbutylazine; cyanazine; hydroxypropazine
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Monoclonal antibodies were raised against seven haptens, representing different structures of the s-triazine group of herbicides, Two side groups on the s-triazine ring were used to couple reactive s-triazine-haptens to immunogenic carrier molecules. For each hapten, one monoclonal antibody was selected for further analysis based on its affinity for the homologous s-triazine. The selected monoclonal antibodies were characterized with respect to their reactivity to the seven immunogens in a direct ELISA assay and cross-reactivity to nine free s-triazines (atrazine, hydroxyatrazine, simazine, hydroxysimazine, terbutylazine, deisopropylatrazine, deethylatrazine, deethylterbutylazine and cyanazine) in competitive assays. The pattern of reactivities illustrates the varying antigenic dominance of the different side groups of the s-triazine ring as well as the potential of monoclonal antibodies for use in screening drinking water, etc, for the presence of s-triazine herbicides. (C) 2001 Elsevier Science B,V. All rights reserved.
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