Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 98, Issue 12, Pages 6605-6610Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.121180398
Keywords
RNase P; tRNA processing
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Funding
- NIAID NIH HHS [T32 AI007210, T32 AI07210-17] Funding Source: Medline
- NIGMS NIH HHS [R01 GM019422, R37 GM019422, GM19422] Funding Source: Medline
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Narrow spectrum antimicrobial activity has been designed to reduce the expression of two essential genes, one coding for the protein subunit of RNase P (C5 protein) and one for gyrase (gyrase A). In both cases, external guide sequences (ECS) have been designed to complex with either mRNA, Using the EGS technology. the level of microbial viability is reduced to less than 10% of the wild-type strain. The EGSs are additive when used together and depend on the number of nucleotides paired when attacking gyrase A mRNA, In the case of gyrase A, three nucleotides unpaired out of a 15-mer EGS still favor complete inhibition by the EGS but five unpaired nucleotides do not.
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