Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 405, Issue 2, Pages 197-203Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.01.008
Keywords
Atherosclerosis; IL-1; Macrophages; Cytokines; ApoE-deficient mice
Categories
Funding
- Sheba Medical Center, Tel Hashomer, Israel
- Sami and Angela Shamoon Vascular Biology Research Fund
- Israel Ministry of Health Chief Scientist's Office [3953]
- NIH [AI-15614]
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Objective: Interleukin (IL)-1 alpha and IL-1 beta are products of macrophages, endothelial cells and vascular smooth muscle cells; moreover, each of these cell types is affected by the pro-inflammatory properties of both IL-1's. Whereas several studies demonstrate the proatherogenic properties of IL-1 beta, the role of IL-1 alpha in atherogenesis remains unclear. We assessed whether IL-1 alpha and IL-1 beta from tissue resident vascular cells or emigrating bone marrow-derived cells promote the development of atherosclerosis in apoE-/- mice and determined the effect of selective macrophage IL-1 alpha or IL-1 beta deficiency on degradation of LDL and cytokine production. Methods: We generated strains of double knock-out (KO) mice (apoE-/-/IL-1 alpha-/- and apoE-/-/IL-1 beta-/-) and created chimeras consisting of apoE-/- mice reconstituted with bone marrow-derived cells from apoE-/-/IL-1+/+, apoE-/-/IL-1 alpha-/- and apoE-/-/IL-1 beta-/-. Results: The areas of aortic sinus lesions were lower in either double KO mice compared to solely apoE-/- mice, despite higher non-HDL cholesterol levels. Importantly, selective deficiency of IL-1 alpha or IL-1 beta in bone marrow-derived cells inhibited atherogenesis to the same extent as in double KO mice without affecting plasma lipids. Aortic sinus lesions in apoE-/- mice transplanted with IL-1 beta-/- or IL-1 alpha-/- cells were 32% and 52% lower, respectively, than in IL-1+/+ transplanted mice. Ex vivo, isolated IL-1 alpha-/- macrophages from atherosclerotic mice degraded LDL and secreted IL-6, TNF alpha and IL-12 similarly to IL-1+/+ macrophages; however, IL-1 alpha deficient macrophages secreted reduced levels of IL-1 beta (-50%) and 2-3-fold higher levels of the anti-inflammatory cytokine IL-10. Conclusion: We show for the first time that it is IL-1 alpha from bone marrow-derived cells that accelerates atherogenesis in apoE-deficient mice rather than constitutive IL-1 alpha in vascular cells, possibly by increasing the inflammatory cytokine profile of macrophages. (C) 2011 Elsevier Inc. All rights reserved.
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