Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 410, Issue 3, Pages 549-554Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.06.022
Keywords
TRIM22; IFN-gamma; BRG1; IRF-1; Recruitment
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Funding
- National Natural Science Foundation of China [30890141, 81072428, 30872355]
- Shanghai STCSM [09JC1401800]
- Ministry of Education of China [2009071120060]
- Major State Basic Research Development Program of China [2007CB512401]
- Jiangsu Provincial Basic Research Program (Innovation Scholars Climbing Program) [SBK201010093]
- Program for Outstanding Medical Academic Leader of Shanghai [LJ06011]
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The modification of chromatin structure is increasingly recognized to be an important facet of transcriptional regulation. Here, we report that Brahma-related gene 1 (BRG1), a chromatin remodeling enzyme, plays a crucial role in IFN-gamma-induced TRIM22 expression. Our results showed that IFN-gamma failed to induce TRIM22 expression in BRG1-deficient SW-13 cells, and reconstitution of BRG1 in this cell line could restore IFN-gamma induction of TRIM22. Furthermore, it was revealed that BRG1 absence, per se, did not impair IFN-gamma-induced IRF-1 expression, but blocked its access to TRIM22 promoter, and BRG1-dependent induction of TRIM22 perfectly correlated with BRG1-dependent recruitment of IRF-1 to TRIM22 promoter. We also found that the DNA-dependent ATPase domain of BRG1 was required for TRIM22 expression and IRF-1 recruitment in response to IFN-gamma stimulation, suggesting that BRG1-mediated chromatin remodeling is critical for the IFN-gamma-inducibility of TRIM22 gene. (C) 2011 Elsevier Inc. All rights reserved.
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