Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 413, Issue 2, Pages 206-211Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.08.059
Keywords
Tandem affinity purification; HP1; Chromodomain-containing CBX proteins; Chromatin
Categories
Funding
- CNRS
- l'Universite de Lille 1 Sciences et Technologies
- Ministere de la Recherche et de l'Enseignement Superieur
- Region Nord-Pas de Calais
- European Regional Developmental Funds through the Contrat de Projets Etat-Region (CPER)
- French Ministere de la Recherche et de l'Enseignement Superieur
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Heterochromatin protein 1 (HP1) has first been described in Drosophila as an essential component of constitutive heterochromatin required for stable epigenetic gene silencing. Less is known about the three mammalian HP1 isotypes CBX1, CBX3 and CBX5. Here, we applied a tandem affinity purification approach coupled with tandem mass spectrometry methodologies in order to identify interacting partners of the mammalian HP1 isotypes. Our analysis identified with high confidence about 30-40 proteins co-eluted with CBX1 and CBX3, and around 10 with CBX5 including a number of novel HP1-binding partners. Our data also suggest that HP1 family members are mainly associated with a single partner or within small protein complexes composed of limited numbers of components. Finally, we showed that slight binding preferences might exist between HP1 family members. (C) 2011 Elsevier Inc. All rights reserved.
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