Journal
ANALYTICAL BIOCHEMISTRY
Volume 293, Issue 2, Pages 194-203Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/abio.2001.5119
Keywords
BIACORE; molecular interactions; binding kinetics; numerical integration; surface plasmon resonance; biosensor; mass transport; complex binding
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Data from real-time molecular interaction analysis using BIACORE are currently evaluated by numerical integration. We have investigated the ability of two software packages (BLCevaluation 3.0 and CLAMP99) to analyze complex interactions. Three experimental data sets of high quality obtained with BIACORE upgraded and 2000 instruments, representative of simple bimolecular, heterogeneous ligand, and mass-transport-limited interactions, were processed by the global fitting procedure. The two software, which differ mainly in the statistical assessment of the output values,:were able to discriminate correctly between various interacting models and provided very close output parameters with satisfactory statistical tests. (C) 2001 Academic Press.
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