Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 404, Issue 1, Pages 250-253Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.11.102
Keywords
Tobacco mosaic virus (TMV) RNA; Omega sequence; Translational enhancers; Sedimentation analysis; Temperature melting
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Funding
- Russian Foundation for basic research [09-04-01729a, 09-04-00537a]
- Presidium of Russian Academy of Sciences
- Russian Federation [8488.2010.4]
- Fogarty International Center [R03TW008217]
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The 5'-untranslated sequence of tobacco mosaic virus RNA - the so called omega leader - is a well-known translational enhancer. The structure of the omega RNA has unusual features. Despite the absence of extensive secondary structure of the Watson-Crick type, the omega RNA possesses a stable compact conformation. The central part of the omega sequence contains many CAA repeats and is flanked by U-rich regions. In this work we synthesized the polyribonucleotides containing modified omega sequences, and studied them using analytical ultracentrifugation and thermal melting techniques. It was demonstrated that changes made in both the central and the 3'-proximal part of the sequence led to a strong destabilization of the omega RNA structure. We conclude that the regular (CAA)(n) core region and the 3'-proximal AU-rich region of the omega RNA interact with each other and contribute together to the formation of a stable tertiary structure. (C) 2010 Elsevier Inc. All rights reserved.
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