Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 406, Issue 1, Pages 20-24Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.01.091
Keywords
Cell culture; alpha-Ketoglutarate; Dulbecco's Modified Eagle's Medium; Hydrogen peroxide; Ascorbate; Epigallocatechin gallate
Categories
Funding
- Tan Chin Tuan Centennial fund
Ask authors/readers for more resources
Ascorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, addition of cc-ketoglutarate, which is known to be released by several cell types, decreased the levels of H2O2, and the alpha-ketoglutarate was depleted and converted to succinate. These observations could account for previous reports of the protective effects of alpha-ketoglutarate in promoting the growth of cells in culture, and may contribute to explaining some of the variability in the literature in reported rates of H2O2 production from autoxidisable compounds in cell culture systems. (C) 2011 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available