4.5 Article

A new caffeine biosynthetic pathway in tea leaves:: utilisation of adenosine released from the S-adenosyl-L-methionine cycle

Journal

FEBS LETTERS
Volume 499, Issue 1-2, Pages 50-54

Publisher

WILEY
DOI: 10.1016/S0014-5793(01)02512-1

Keywords

caffeine biosynthesis; S-adenosyl-L-methionine; adenosine; S-adenosyl-L-homocysteine; subcellular compartmentation

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The four-step caffeine biosynthetic pathway includes three methylation steps that utilise S-adenosyl-L-methionine (SAM) as the methyl donor, In the process SAM is converted to S-adenosyl-L-homocysteine (SAH) which in turn is hydrolysed to L-homocysteine and adenosine, Significant amounts of radioactivity from [methyl-C-14]methionine and [methyl-C-14]SAM were incorporated into theobromine and caffeine in young tea leaf segments, and very high SAH hydrolase activity was found in cell-free extracts from young tea leaves. Substantial amounts of radioactivity from [adenosyl-C-14]SAH were also recovered as theobromine and caffeine in tea leaf segments, indicating that adenosine derived from SAH is utilised for the synthesis of the purine ring of caffeine, From the profiles of activity of related enzymes in tea leaf extracts, it is proposed that the major route from SAM to caffeine is a SAM --> SAH --> adenosine --> adenine --> AMP --> IMP --> XMP --> xanthosine --> 7-methylsanthosine --> 7-methylxanthine --> theobromine --> caffeine pathway. In addition, direct adenosine kinase-catalysed formation of AMP from adenosine may participate as an alternative minor route, The activity of two of the three N-methyltransferase activities involved in caffeine biosynthesis and part of the activities of SAH hydrolase, adenosine nucleosidase, adenine phosphoribosyltransferase and adenosine kinase were located in tea chloroplasts, In contrast, no detectable activity of SARI synthetase was associated with the purified chloroplast fraction. This is a first demonstration that the purine skeleton of caffeine is synthesised from adenosine released from the SAM cycle. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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