Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 276, Issue 25, Pages 22779-22787Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M101436200
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Funding
- NHLBI NIH HHS [HL56386] Funding Source: Medline
- NIAID NIH HHS [AI22571, AI20241, AI41995] Funding Source: Medline
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Eosinophils and basophils, when activated, become major sources of cysteinyl leukotrienes, eicosanoid mediators pertinent to allergic inflammation. We show that the C-C chemokines, eotaxin and RANTES (regulated upon activation normal T cell expressed and secreted), activate eosinophils and basophils for enhanced leukotriene C-4 (LTC4) generation by distinct signaling and compartmentalization mechanisms involving the induced formation of new cytoplasmic lipid body organelles, Chemokine-induced lipid body formation and enhanced LTC4 release were both mediated by CCR3 receptor G protein-linked downstream signaling involving activation of phosphoinositide 3-kinase, extracellular signal-regulated kinases 1 and 2, and p38 mitogen-activated protein kinases. Chemokine-elicited lipid body numbers correlated with increased calcium ionophore-stimulated LTC4 production and as demonstrated by intracellular immunofluorescent localization of newly formed eicosanoid, lipid bodies were the predominant sites of LTC4 synthesis in both chemokine-stimulated eosinophils and chemokine-primed and ionophore-activated eosinophils. Eotaxin and RANTES initiated signaling via phosphoinositide S-kinase and mitogen-activated protein kinases both elicits the formation of lipid body domains and promotes LTC4 formation at these specific extranuclear sites.
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