Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 406, Issue 1, Pages 85-88Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.01.115
Keywords
Vinculin; Head-tail interaction; Mutant A50I; Focal adhesions; Actin cytoskeleton; Magnetic tweezers; Traction microscopy; Immunofluorescence
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Funding
- Bayerisch-Franzosisches Hochschulzentrum
- Deutscher Akademischer Austausch Dienst
- Bavaria California Technology Center
- Deutsche Forschungsgemeinschaft
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This study evaluates the influence of vinculin in closed conformation on the mechanical properties of cells. We demonstrate that MEFvin(-/-) cells transfected with the eGFP-vinculin mutant A50I (talin-binding-deficient-vinculin in a constitutively closed conformation) show 2-fold lower stiffness and focal adhesion density compared to MEFvin(+/+) and MEFRRescue cells. MEFA50I I cells are as stiff as MEFvin-/- cells with similar focal adhesion density. Further, 2D traction microscopy indicates that MEFA50I and MEFvin(-/-) cells generate 3- to 4-fold less strain energy than MEFvin(+/+) and MEFRescue cells. These results demonstrate that vinculin's mechano-coupling function is dependent on its conformational state. (C) 2011 Elsevier Inc. All rights reserved.
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