Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 414, Issue 2, Pages 412-418Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.09.103
Keywords
Microgravity; p38; Rotary cell culture system; Adipose derived stem cells
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Funding
- National Natural Science Foundation of China [30973038, 81001529]
- Natural Science Foundation of Shandong Province [Q2008C18]
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Mesenchymal stem cells (MSCs) are multi-potent, and the chondrogenesis of MSCs is affected by mechanical stimulation. The aim of this study was to investigate, using a rotary cell culture system (RCCS) bioreactor, the effects of microgravity on the chondrogenic differentiation of human adipose-derived MSCs (ADSCs), which were cultured in pellets with or without the chondrogenic growth factor TGF-beta 1. In addition, we evaluated the role of the p38 MAPK pathway in this process. The real-time PCR and histological results show that microgravity has a synergistic effect on chondrogenesis with TGF-beta 1. The p38 MAPK pathway was activated by TGF-beta 1 alone and was further stimulated by microgravity. Inhibition of p38 activity with 5B203580 suppressed chondrocyte-specific gene expression and matrix production. These findings suggest that the p38 MAPK signal acts as an essential mediator in the microgravity-induced chondrogenesis of ADSCs. (C) 2011 Elsevier Inc. All rights reserved.
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