Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 413, Issue 4, Pages 515-520Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.08.124
Keywords
Nuclear hormone receptor; Caenorhabditis elegans; NHR-23; Transcription; Gene expression; Development; Hedgehog; Molting; ROR
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Funding
- Ministry of Education, Youth and Sports of the Czech Republic [0021620806]
- Czech Science Foundation [304/08/0970]
- Charles University in Prague [SVV262502]
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) of the National Institutes of Health, USA
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NHR-23, a conserved member of the nuclear receptor family of transcription factors, is required for normal development in Caenorhabditis elegans where it plays a critical role in growth and molting. In a search for NHR-23 dependent genes, we performed whole genome comparative expression microarrays on both control and nhr-23 inhibited synchronized larvae. Genes that decreased in response to nhr-23 RNAi included several collagen genes. Unexpectedly, several hedgehog-related genes were also down-regulated after nhr-23 RNAi. A homozygous nhr-23 deletion allele was used to confirm the RNAi knockdown phenotypes and the changes in gene expression. Our results indicate that NHR-23 is a critical co-regulator of functionally linked genes involved in growth and molting and reveal evolutionary parallels among the ecdysozoa. (C) 2011 Elsevier Inc. All rights reserved.
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